The painting depicts a remarkable iconographic theme common in the Christian tradition. The Virgin Mary and her divine son, whom she holds in her arms, are the main characters in this scene, also known as the "Sacred Conversation". The nudity of the little Jesus is of particular note, revealing his earthly nature and complementing his divine nature. Interesting is the unusual presence of an older woman in the background, traditionally identified as St. Elizabeth, the mother of John the Baptist, but could possibly be St. Anne, the mother of the Virgin Mary. This painting likely depicts St. Anne as she is holding a book. Anne extends a goldfinch to the Messiah because, according to Christian belief, the red spot on the bird's head appeared during Jesus' ascent to Golgotha, when the bird pulled a thorn from the Saviour's crown and stained its feathers with blood. Mary points to the goldfinch with her right finger, emphasising the importance of this symbolic element in the painting.