In the sixteenth century, Italy witnessed changes in the iconographic representation of the Virgin Mary with Child. Their figures became half-length, often accompanied by Saint John and Saint Joseph depictions. In the painting, the infant Jesus is holding onto the Madonna. On the right is little John the Baptist, looking towards Mary, wearing a cilice. On the other side is Saint Joseph, looking reverently at Jesus. Mannerism had several primary visual sources of formation, namely the works of Michelangelo and Raphael, whose features Perino del Vaga skilfully adopted and synthesised.