In the painting, Luca Giordano illustrated an episode from the apocryphal addition to the Old Testament Book of Daniel. It is the story of two lecherous elders who lusted after Susanna, a beautiful young woman. They hid in the garden where she had come to bathe and threatened to accuse her of adultery, a crime punishable by death if she refused to submit to their desires. The painting portrays the moment when Susanna is undressing, and the men approach and address her. Susanna looks shocked and tries to cover her breasts with her hand. She was a virtuous wife and rejected the advances of the men, who then carried out their threat. Later, she was accused of adultery, but her innocence was proven when her accusers were subjected to cross-examination and caught in a lie. The shades of the male figures are of a dark earthy colour, almost identical to their robes, which starkly contrasts with the pale cream hue of Susanna's skin. The bright paleness of the woman's body might imply her innocence and purity. This theme was popular among commissioners, as it offered a justification for depicting a partially clothed female figure that could be openly admired.