In the painting, against the backdrop of a dark wall, figures of Madonna and Saint Anne are depicted sitting. Madonna is shown in profile, with her head slightly turned to the right. The woman's gaze is directed at the child she holds on her lap, supporting his foot with her right hand. Madonna is wearing a brown dress with a white collar, a white headscarf, and a dark cloak on her lap. The child is turned towards Saint Anne, facing left in three-quarters view. She is dressed in a white headscarf and a green dress, with a red cloak draped over her knees. In the upper right corner is an architectural landscape in the Renaissance style. The depiction of Mary with Child Christ and Saint Anne, created by Leonardo da Vinci, became widespread. The cult of St Anne, the mother of the Virgin Mary, reached the West with Christian refugees fleeing from Muslim conquerors. The very first image of her appeared in the Santa Maria Antiqua church in Rome, where she is portrayed with the Mother of God. Around the fourteenth century, St Anne was a popular figure, partly because her late motherhood confirmed the doctrine of the Immaculate Conception of the Virgin Mary. According to apocryphal tradition, Anne was of the lineage of King David and was the daughter of the priest Matan from the lineage of Judah. She was also the aunt of St Elizabeth, the mother of St John the Baptist. Anne was childless, which was considered a disgrace among the people. Together with her husband Joachim, she fervently prayed to God for a child. One day, an angel of the Lord appeared to her, bringing the joyous news that her prayer had been heard. God fulfilled his promise when, after a long wait, the couple finally held their child – Mary, the mother of Jesus Christ.